【脂質代謝分析】Lipid Peroxidation (MDA) Assay kit，K739-100, 100 assays

Lipid Peroxidation (MDA) Assay Kit Catalog#: K739-100 | Size: 100 assays 【供應廠商】萊恩生物科技有限公司 【公司電話】04-2523-9639 【業務專員】陳俊豪 0937-229803 【電子信箱】biolion0819@gmail.com

Kit Summary:
• Detection method-Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm)
• Sample type- Cell and Tissue culture supernatants, plasma and other biological fluids (optimized by end user).
• Species reactivity- All
• Applications- The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Features & Benefits:
• Simple procedure
• Fast and convenient
• Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples
Kit Components:
• MDA Lysis Buffer
• Phosphotungstic Acid Solution
• BHT (100X)
• TBA
• MDA Standard (8.34M)
Description:
Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. BioVision’s Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Catalog #	Product Name+	Size
K610-100	Adipogenesis Assay Kit	100 assays
K4901-100	Adiponectin (human) Elisa Assay Kit	96 assays
K4902-100	Adiponectin (mouse) Elisa Assay Kit	96 assays
K4903-100	Adiponectin (rat) Elisa Assay Kit	96 assays
K632-100	beta-Hydroxybutyrate (β-HB) Assay Kit	100 assays
K601-100	CETP Activity Assay Kit	100 assays
K602-100	CETP Inhibitor Drug Screening Kit	100 assays
K603-100	Cholesterol/Cholesteryl Ester Quantitation Kit	100 assays
K615-100	Choline/Acetylcholine Quantification Kit	100 assays
K612-100	Free Fatty Acid Quantification Kit	100 assays
K630-100	Free Glycerol Assay Kit	100 assays
K606-100	Glucose Assay Kit	100 assays
K613-100	HDL and LDL/VLDL Quantification Kit	100 assays
K722-100	Lipase Activity Assay Kit	100 assays
K576-100	Phosphatidylcholine Assay Kit	100 assays
K705-400	PhosphoSeek™ PDE5A Assay Kit	400 assays
K708-400	PhosphoSeek™ Sphingosine Kinase Assay Kit	400 assays
K604-100	PLTP Activity Assay Kit	100 assays
K605-100	PLTP Inhibitor Drug Screening Kit	100 assays
K622-100	Triglyceride Quantification Kit	100 assays
K4907-100	Visfatin (human) Elisa Assay Kit	100 assays

乳酸偵測分析套組Lactate Assay Kit, K607-100。利用核磁共振檢測大腦中乳酸鹽含量，可瞭解人類大腦的老化程度；發現乳酸鹽含量越高，大腦越老化。BIOVISION呈色/螢光乳酸分析

乳酸偵測分析套組/Lactate Assay Kit/K607-100
Catalog#: K607-100 | Size: 100 assays

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

 

背景資料	乳酸鹽濃度的異常升高與多種疾病如糖尿病、酸毒症等疾病有密切的關係。L(+)-乳酸鹽是人體中乳酸鹽代謝的主要中間產物，並且存在於血液中。D(-)-乳酸鹽也存在，但含量很低，只有L(+)-乳酸鹽 的1-5%。過高的乳酸鹽濃度提示糖尿病和乳酸性酸毒症等疾病的存在。本試劑盒檢測L(+)-乳酸鹽的濃度。近期也有研究顯示，利用核磁共振檢測大腦中乳酸鹽含量，可瞭解人類大腦的老化程度；並發現乳酸鹽含量越高，大腦越老化。
產品描述	Biovision的乳酸檢測分析試劑盒提供了一種簡單、快速方便的方法來檢測生物學標本（人血液、細胞、培養基、發酵培養基等）中 L(+)-Lactate 的含量的方案，並且無需對標本進行預處理。在該乳酸鹽檢測試劑盒分析中，乳酸鹽特異性的與酶混合物反應並形成產物，該產物與乳酸鹽探針互相作用並產生顏色（λ= 570 nm）或者螢光（Ex/Em = 535/587 nm）。試劑盒檢測範圍為含0·001 -10 mM 乳酸鹽樣本。
產品特點	1、步驟簡單，只需要大約40分鐘即可完成檢測；2、操作快速並且方便；3、試劑盒內包括所有精確檢測乳酸鹽含量的必需組分
保存建議	-20℃保存
其他	BioVision的公司總部在美麗的三藩市灣地區，它是全球生命科學公司的領導者。BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力。包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因數和酶等多種產品，以及其他創新的研究工具。其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖症、細胞增殖與毒性、信號轉導、幹細胞研究等領域。作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務品質。

相關產品	D-乳酸檢測分析試劑盒(比色法) 乳酸檢測分析試劑盒II LDH乳酸脫氫酶活性定量試劑盒 乳酸檢測分析試劑盒 【LDHB】重組人乳酸脫氫酶-B

【細胞培養液的條件】

Lactate分析，細胞培養的條件，可直接測量含有FBS的培養液。

(全部實驗都含有FBS)

以A549細胞株為例，每24well中的細胞數為5X104，24well中加入500ul含有FBS的DMEM培養液，培養隔夜後，fresh培養液並取細胞培養液直接測量Lactate含量。

PS. 1ul至10ul DMEM測試，10ul會使呈色變色~

Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect 0.001 -10 mM of various Lactate samples.

Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of Lactate colorimetrically and fluorometrically

Kit components:

• Lactate Assay Buffer
• Lactate Probe (in DMSO)
• Lactate Enzyme Mix
• L(+)-Lactate Standard (100 nmol/µl)

Description:
Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples. The kit can detect 0.001 -10 mM of various Lactate samples.

Storage Conditions:
-20°C

血清、血漿、培養液中, 游離脂肪酸定量套組Free Fatty Acid Quantification Kit,K612-100 BIOVISION代理

游離脂肪酸定量套組
Free Fatty Acid Quantification Kit
Catalog#: K612-100 | Size: 100 assays

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

產品特點:

(一)靈敏度高 (偵測長鏈游離脂肪酸)

脂肪酸氧化作用的循環，每經過一次循環中四個酵素的作用，就釋出一個兩碳的acetyl CoA衍生物,藉由螢光或是冷光儀可偵測長鏈脂肪酸的含量或脂肪酸氧化的程度.

(二) 應用範圍廣: 可直接應用在血清、血漿、 體液、食物、及培養液諸多種類等

(三) 操作方便:

(1) 準備標準曲線

(2) 將預測的sample (如液狀樣品: 血清 血漿體液 培養液)以assay buffer調整至50ul/well

(3) 加入2ul ACS Reagent 於標準液及sample 混合均勻後於室溫37度培養30分鐘

(4) 預混50 μl Reaction Mix, 並各別添加至ACS反應好的標準液及sample混合均勻後於室溫37 度培養30分鐘(避光)

(5) Measure O.D. 570 nm for colorimetric assay or fluorescence at

Ex/Em = 535/590 nm in a micro-plate reader.

(6)計算fatty acid含量

Published References:

1) Bobrovnikova-Marjon E et al (2008) PNAS; 105: 16314 - 16319.

Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Application- The kit has a detection limit 2 µM free fatty acid in variety samples. Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples Kit components:
• Fatty Acid Assay Buffer
• Fatty Acid Probe (lyophilized)
• Dimethylsulfoxide (DMSO; Anhydrous)
• ACS Reagent
• Enzyme Mix
• Enhancer
• Palmitic Acid Standard (1nmol/µl) Description:
Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 µM free fatty acid in variety samples. Storage Conditions:
-20°C

病人尿液及血液中Creatine含量分析，BIOVISION Creatine Assay Kit，#k635-100; Creatinine assay kit,#K625-100

Creatine Assay Kit
Catalog#: K635-100 | Size: 100 assays

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

Catalog#	Product Name	Size
K635-100	Creatine Assay Kit	100 assays
K625-100	Creatinine Assay Kit	100 assays
K636-100	Sarcosine Assay Kit	100 assays

Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications-The kit can detect 0.001 – 10 mM Creatine.
Features & Benefits:
• Simple procedure; takes ~ 1 hour
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Creatine
Kit components:
• Creatine Assay Buffer
• Creatine Probe
• DMSO (Anhydrous)
• Creatinase
• Creatine Enzyme mix
• Creatine Standard (10 µmol)
Description:
Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development. BioVision’s Creatine Assay Kit provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (λmax = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001 – 10 mM Creatine.

1. 尿液及血液中Creatine分析

(1). 方法參照BIOVISION套組 (貨號#K635-100, 太鼎生技)。以570nm波段測量，得到O.D值 (如表2所示)。

(2). 每個O.D.值，需扣除background值 (如表3所示)。(此backgroung control為參照BIOVISION套組內建議)

*Note: Sarcosine gives background for the assay. For samples which may contain a significant amount of sarcosine, do a background control. Prepare a reaction without the creatinase (replacing the creatinase with 2 μl assay buffer).

(3). 以迥歸曲線換算，得到Creatine含量，其單位以nmol表示，其線性範圍介於0~10nmol (如表4所示)。

(4). 換算出Creatine (nmol) 含量後，此Creatine (nmol)含量是較高的，因為尿液及血液樣品，全部經由10Kd spin column濃縮過，所以最後到的Creatine (nmol)讀值會比較高，正確讀值需再除以濃縮倍數，才得到正確的Creatine含量nmol。

因為Creatine含量再除以濃縮倍數後，其讀值會太小，線性範圍 (0~10nmol)，所以最後以最終濃度uM或mM來表示(如表5所示)，最終濃度換算公式如下。

樣品Creatine含量 (nmol) / 50ul = mM

樣品Creatine含量 (nmol) / 50ul *1000 = uM

因為標準品的最終濃度為0~200uM (理論上的值), 所以採用uM為單位

實驗上測出算出標準品的最終濃度為-8.63306~ 189.884uM。

Adiponection於細胞、血液、血漿內含量測量。Adiponectin (human) Elisa Assay Kit, k4901-100

Adiponectin (human) Elisa Assay Kit
Catalog#: K4901-100 | Size: 96 assays

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

 

樣品來源: 細胞、組織培養上清液、尿液、血漿、及血清皆可以測量

應用於哺乳類動物

抗體直接coating於孔盤，快速測量樣品內Adiponectin含量

Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants
Features & Benefits:
• Simple procedure
• Fast and convenient
Kit components:
• Antibody coated 96-well plate (12 x 8-well strips)
• 5X Wash concentrate, 100 ml
• 5X Diluent, 50 ml
• Secondary antibody, 12 ml
• 100X Detector, 150 µl
• Standard, recombinant human adiponectin (64 ng), 1 vial, lyophilized
• QC sample= positive control having 7-11 µg/ml human adiponectin, 1 vial, lyophilized
• Substrate I, 6 ml
• Substrate II, 6 ml
• Stop solution, 12 ml
Description:
Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for human adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-human adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
Adiponectin : 是專門由脂肪組織所分泌出來的一種在人體內含量很豐富的蛋白質。在脂肪細胞分化的過程中， adiponectin 的訊息核醣核酸(messenger RNA, mRNA)的含量，會被誘導合成約100倍以上。Adiponectin之分子量大約是30 kDa左右，在成年人類的血漿濃度約為2-20 μg/mL。雖然它是由脂肪組織所分泌，直覺上會以為脂肪組織愈多的人，體內濃度應該愈高，但事實上卻剛好相反。在肥胖的健康人體內的adiponectin濃度，遠比非肥胖者為低

Hydroxyproline 或4-hydroxyproline分析套組，測量組織、細胞懸浮液、尿液、血漿及血清內的4-羥基脯胺酸含量，K555-100，BIOVISION代理商。

Hydroxyproline Assay Kit
Catalog#: K555-100 | Size: 100 assays

Hydroxyproline 或4-hydroxyproline分析套組，測量組織、細胞懸浮液、尿液、血漿及血清內的4-羥基脯胺酸含量，K555-100，BIOVISION代理商。
sales:

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

4-羥基脯胺酸 (4-hydroxyproline) 是脯胺酸的衍生物和 5-羥基離胺酸 (5-hydroxylysine)，二者可見於結締組織的膠原蛋白中 (collagen)。

Hydroxyproline (4-hydroxyproline, 4-羥基脯胺酸) is a common nonproteinogenic amino acid. It is found only in collagen and elastin in mammals but exists in a number of other proteins in plants. Hydroxyproline is formed only as a post-translational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline. Hydroxyproline in tissue hydrolysates is a direct measure of the amount of collagen or gelatin present. A variety of disease states are believed to affect collagen turnover and can cause elevated serum or urine hydroxyproline. Such conditions range from neoplastic, inflammatory, renal or bone disease to endocrine and autoimmune disorders. BioVision’s Hydroxyproline Assay Kit is designed to measure hydroxyproline in tissue or protein/peptide hydrolysates. It can be used to measure hydroxyproline from other biological samples such as serum or urine if they have undergone a prior purification process. It is an easy, convenient method which results in a chromogen with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-2 μg.
Kit Summary:
• Detection method- Absorbance (560 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The assay is useful over the range of 0.1-2 μg.
Features & Benefits:
• Simple procedure; takes less than ~100 minutes
• Convenient and easy to use
Kit components:
• Oxidation Buffer
• Chloramine T Concentrate
• Perchloric acid/Isopropanol Solution
• DMAB Concentrate (in DMSO)
• Hydroxyproline Standard (1 mg/ml)
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
一般操作注意事項:
Store the kit at +4°C and protect from light. Please read the entire protocol before performing the assay. The reagent concentrates are stable as supplied. Once the concentrates have been diluted to working concentration, they are only good for 2-3 hr so only make as enough each reagent as necessary for the number of samples and standards to be quantified.

Adiponection於細胞、血液、血漿內含量測量。Adiponectin (human) Elisa Assay Kit, k4901-100

Adiponectin (human) Elisa Assay Kit
Catalog#: K4901-100 | Size: 96 assays

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

 

樣品來源: 細胞、組織培養上清液、尿液、血漿、及血清皆可以測量

應用於哺乳類動物

抗體直接coating於孔盤，快速測量樣品內Adiponectin含量

Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants
Features & Benefits:
• Simple procedure
• Fast and convenient
Kit components:
• Antibody coated 96-well plate (12 x 8-well strips)
• 5X Wash concentrate, 100 ml
• 5X Diluent, 50 ml
• Secondary antibody, 12 ml
• 100X Detector, 150 µl
• Standard, recombinant human adiponectin (64 ng), 1 vial, lyophilized
• QC sample= positive control having 7-11 µg/ml human adiponectin, 1 vial, lyophilized
• Substrate I, 6 ml
• Substrate II, 6 ml
• Stop solution, 12 ml
Description:
Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for human adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-human adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
Adiponectin : 是專門由脂肪組織所分泌出來的一種在人體內含量很豐富的蛋白質。在脂肪細胞分化的過程中， adiponectin 的訊息核醣核酸(messenger RNA, mRNA)的含量，會被誘導合成約100倍以上。Adiponectin之分子量大約是30 kDa左右，在成年人類的血漿濃度約為2-20 μg/mL。雖然它是由脂肪組織所分泌，直覺上會以為脂肪組織愈多的人，體內濃度應該愈高，但事實上卻剛好相反。在肥胖的健康人體內的adiponectin濃度，遠比非肥胖者為低