游離脂肪酸定量套組
Free Fatty Acid Quantification Kit
Catalog#: K612-100 | Size: 100 assays
【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
產品特點:
(一)靈敏度高 (偵測長鏈游離脂肪酸)
脂肪酸氧化作用的循環，每經過一次循環中四個酵素的作用，就釋出一個兩碳的acetyl CoA衍生物,藉由螢光或是冷光儀可偵測長鏈脂肪酸的含量或脂肪酸氧化的程度.
(二) 應用範圍廣: 可直接應用在血清、血漿、 體液、食物、及培養液諸多種類等
(三) 操作方便:
(1) 準備標準曲線
(2) 將預測的sample (如液狀樣品: 血清 血漿體液 培養液)以assay buffer調整至50ul/well
(3) 加入2ul ACS Reagent 於標準液及sample 混合均勻後於室溫37度培養30分鐘
(4) 預混50 μl Reaction Mix, 並各別添加至ACS反應好的標準液及sample混合均勻後於室溫37 度培養30分鐘(避光)
(5) Measure O.D. 570 nm for colorimetric assay or fluorescence at
Ex/Em = 535/590 nm in a micro-plate reader.
(6)計算fatty acid含量
Published References:
1) Bobrovnikova-Marjon E et al (2008) PNAS; 105: 16314 - 16319.
Kit Summary: • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Application- The kit has a detection limit 2 µM free fatty acid in variety samples. Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples Kit components:
• Fatty Acid Assay Buffer
• Fatty Acid Probe (lyophilized)
• Dimethylsulfoxide (DMSO; Anhydrous)
• ACS Reagent
• Enzyme Mix
• Enhancer
• Palmitic Acid Standard (1nmol/µl) Description:
Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 µM free fatty acid in variety samples. Storage Conditions:
-20°C
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