Triglyceride Quantification Kit三酸甘油酯定量套組, 100個分析, 可應用於組織樣品脂肪定量, 以吸光值或螢光讀值。靈敏度2 pmol~10nmol範圍。
【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
三酸甘油酯定量套組, 訂購貨號Catalog#: K622-100 | Size: 100 assays
【組織樣品準備】
For tissue(~100 mg),cells (~10 million) or other non-aqueous samples, homogenize in 1 ml solution containing 5 % NP-40 in water, slowly heat the samples to 80-100 °C in a water bath for 2-5 min or until the NP-40 becomes cloudy, then cool down to room temperature. Repeat the heating one more time to solubilize all triglyceride. Centrifuge for 2 min (top speed using a microcentrifuge) to remove any insoluble material. Dilute
10 fold with dH2O before the assay.
產品介紹
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
產品特點
• Simple procedure; takes ~1 hour
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of triglyceride in various biological samples
產品特點
•Triglyceride Assay Buffer
• TriglycerideProbe (in DMSO, anhydrous)
• Lipase
• TriglycerideEnzyme Mix (lyophilized)
• Triglyceride Standard (1 mM)
Description:
Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. TG are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. Theglycerol is then oxidized to generate a product which reacts with theprobe to generate color (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
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