K555-100 羥脯氨酸分析試劑組 Hydroxyproline Colorimetric Assay Kit
http://www.biovision.com/hydroxyproline-colorimetric-assay-kit-4386.html
主要用來測定組織或蛋白質/多肽水解物中的羥脯氨酸, 亦可用再測定其他的生物樣品，如血清或尿液中的羥脯氨酸含量.
羥脯氨酸是膠原蛋白的組成成分，占其氨基酸總量的13%，膠原蛋白是構成結締組織的主要成分，也是人體內含量最多的蛋白質，當體內結締組織較大量增生或破壞時，如嚴重骨折、燒傷、重癥肺結核和肝硬變、Hodgkin’s病、甲狀腺功能亢進、羥脯氨酸血癥均可造成血、尿中羥脯氨酸含量的增加。
Kit Summary:
• Detection method- Absorbance (560 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The assay is useful over the range of 0.1-2 μg.
Features & Benefits:
• Simple procedure; takes less than ~100 minutes
• Convenient and easy to use
Kit components:
• Oxidation Buffer
• Chloramine T Concentrate
• Perchloric acid/Isopropanol Solution
• DMAB Concentrate (in DMSO)
• Hydroxyproline Standard (1 mg/ml)
Description:
Hydroxyproline (4-hydroxyproline) is a common nonproteinogenic amino acid. It is found only in collagen and elastin in mammals but exists in a number of other proteins in plants. Hydroxyproline is formed only as a post-translational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline. Hydroxyproline in tissue hydrolysates is a direct measure of the amount of collagen or gelatin present. A variety of disease states are believed to affect collagen turnover and can cause elevated serum or urine hydroxyproline. Such conditions range from neoplastic, inflammatory, renal or bone disease to endocrine and autoimmune disorders. BioVision’s Hydroxyproline Assay Kit is designed to measure hydroxyproline in tissue or protein/peptide hydrolysates. It can be used to measure hydroxyproline from other biological samples such as serum or urine if they have undergone a prior purification process. It is an easy, convenient method which results in a chromogen with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-2 μg.
Storage Conditions:
+4°C
